Match details

Record ID: NRN_AX302480
Database: nrnl1 Go to original record
Description: Sequence 12 from Patent WO0177350.
Taxon: unidentified
Original size: 6309 bp
Patents: WO0177350 (lens, espacenet)

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Method: QL-ELE-00-006 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (L 00.00-31, 1998).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 1 344 522 - 179 bp

Amplicon: 179 bp
1     aatcctgttg ccggtctgtc gatgattatc atataatttc tgttgaatta
51    cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
101   gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
151   aacaaaatat agcgcgcaaa ctaggataa 

View other 11 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 17 bp 0 0 1 17 3.95e-07
primerB 100.0 20 bp 0 0 179 160 8.49e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.