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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_AX765962
Database: nrnl1 Go to original record
Description: Sequence 48 from Patent WO02097097. Sequence 54 from Patent WO2004020642. Sequence 48 from patent US 7172881. Sequence 54 from patent US 7262338. Sequence 48 from patent US 8030470. Sequence 48 from Patent EP2123763.
Taxon: unidentified
Original size: 5511 bp
Patents: WO02097097 (lens, espacenet) WO2004020642 (lens, espacenet) US7172881 (lens, espacenet) US2003167535 (lens, espacenet) US7262338 (lens, espacenet) US2004010821 (lens, espacenet) US8030470 (lens, espacenet) EP2123763 (lens, espacenet)

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Method: QL-ELE-00-013 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim T-nos) (Waiblinger et al., 2008).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 4702 4785 + 84 bp
Amplicon: 84 bp 
1     catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
51    cgcaattata catttaatac gcgatagaaa acaa

View other 30799 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 25 bp 0 0 1 25 9.20e-12
primerB 100.0 25 bp 0 0 84 60 9.20e-12
probe 100.0 28 bp 0 0 28 55 2.28e-13

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.