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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_A60108
Database: nrnl1 Go to original record
Description: Sequence 1 from Patent WO9706267. Sequence 1 from Patent EP0757102. Sequence 1 from Patent WO0141558. Sequence 1 from Patent EP1496125. Sequence 1 from patent US 6074876. Sequence 1 from patent US 6506963. Sequence 1 from patent US 6563026. GENETIC TRANSFORMATION USING A PARP INHIBITOR. Sequence 1 from patent US 7659095. Sequence 1 from patent US 8026352. Sequence 1 from patent US 8309699.
Taxon: unidentified
Original size: 4946 bp
Patents: WO9706267 (lens, espacenet) EP0757102 (lens, espacenet) WO0141558 (lens, espacenet) EP1496125 (lens, espacenet) US6074876 (lens, espacenet) US6506963 (lens, espacenet) US6506963 (lens, espacenet) US6563026 (lens, espacenet) US2001029620 (lens, espacenet) JP2007089587 (lens, espacenet) US7659095 (lens, espacenet) US8026352 (lens, espacenet) US8309699 (lens, espacenet)

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Method: QL-ELE-00-026 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of pat gene; bar gene (partim bar) (Debode et al., 2016).
Target:bar

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 733 792 - 60 bp
Amplicon: 60 bp 
1     acaagcacgg tcaacttccg taccgagccg caggaaccgc aggagtggac
51    ggacgacctc

View other 5085 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 1.06e-08
primerB 100.0 18 bp 0 0 60 43 3.56e-08
probe 100.0 18 bp 0 0 21 38 3.56e-08

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.