Match details
Record ID: NRN_AX004880
Database:
nrnl1
Go to original record
Description:
Sequence 1 from Patent WO9910512. Sequence 1 from patent US 6140553. Sequence 1 from patent US 6372963. Sequence 2 from patent US 6483013. Sequence 1 from patent US 6323396. Improved Agrobacterium tumefacience-mediated transformation method of plants. JP 2000509612-A/1: Improved transformation method for plants.
Taxon:
unidentified
Original size:
2345 bp
Patents:
WO9910512 (lens , espacenet )
US6140553 (lens , espacenet )
US6372963 (lens , espacenet )
US6372963 (lens , espacenet )
US6483013 (lens , espacenet )
US6483013 (lens , espacenet )
US6323396 (lens , espacenet )
US6323396 (lens , espacenet )
JP2001514009 (lens , espacenet )
JP2000509612 (lens , espacenet )
View other 15 matches for this record
Method: QL-ELE-00-012
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim CaMV P-35S) (Waiblinger et al., 2008).
Target: CaMV P-35S
View other 15625 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
1189
1270
+
82 bp
1 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51 cgtggaaaaa gaagacgttc caaccacgtc tt
View other 26489 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
18 bp
0
0
1
18
4.54e-08
primerB
100.0
22 bp
0
0
82
61
3.55e-10
probe
100.0
22 bp
0
0
21
42
3.55e-10
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
