JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_AX004880
Database:	nrnl1	Go to original record
Description:	Sequence 1 from Patent WO9910512. Sequence 1 from patent US 6140553. Sequence 1 from patent US 6372963. Sequence 2 from patent US 6483013. Sequence 1 from patent US 6323396. Improved Agrobacterium tumefacience-mediated transformation method of plants. JP 2000509612-A/1: Improved transformation method for plants.
Taxon:	unidentified
Original size:	2345 bp
Patents:	WO9910512 (lens, espacenet) US6140553 (lens, espacenet) US6372963 (lens, espacenet) US6372963 (lens, espacenet) US6483013 (lens, espacenet) US6483013 (lens, espacenet) US6323396 (lens, espacenet) US6323396 (lens, espacenet) JP2001514009 (lens, espacenet) JP2000509612 (lens, espacenet)

Method:	QL-ELE-00-009	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of nopaline synthase terminator (Lipp et al.,2001).
Target:	T-nos

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2132	2249	+	118 bp

Amplicon:

118 bp

1     gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
51    tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
101   attatcgcgc gcggtgtc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	24 bp	0	0	1	24	4.22e-11
primerB	100.0	24 bp	0	0	118	95	4.22e-11

Match details