Match details

Record ID: NRN_AX765954
Database: nrnl1 Go to original record
Description: Sequence 40 from Patent WO02097097. Sequence 46 from Patent WO2004020642. Sequence 40 from patent US 7172881. Sequence 46 from patent US 7262338. Sequence 40 from patent US 8030470. Sequence 40 from Patent EP2123763.
Taxon: unidentified
Original size: 5247 bp
Patents: WO02097097 (lens, espacenet) WO2004020642 (lens, espacenet) US7172881 (lens, espacenet) US2003167535 (lens, espacenet) US7262338 (lens, espacenet) US2004010821 (lens, espacenet) US8030470 (lens, espacenet) EP2123763 (lens, espacenet)

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Method: QL-ELE-00-008 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase promoter.
Target:P-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 204 297 + 94 bp

Amplicon: 94 bp
1     aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt
51    cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctga 

View other 3895 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 26 bp 0 0 1 26 3.03e-12
primerB 100.0 24 bp 0 0 94 71 3.54e-11
probe 100.0 26 bp 0 0 28 53 3.03e-12


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.