Match details

Record ID: NRN_AX167655
Database: nrnl1 Go to original record
Description: Sequence 30 from Patent WO0144457. Sequence 30 from Patent EP1632576. Sequence 30 from patent US 6462258. Sequence 30 from patent US 6660911. Sequence 30 from patent US 6919495. Sequence 30 from patent US 6949696. Sequence 30 from patent US 7112725. Sequence 30 from patent US 7141722. JP 2003516753-A/30: DNA CONSTRUCTS FOR EXPRESSION OF HETEROLOGOUS POLYPETIDES IN PLANTS. Sequence 30 from Patent EP2339005.
Taxon: unidentified
Original size: 1742 bp
Patents: WO0144457 (lens, espacenet) EP1632576 (lens, espacenet) US6462258 (lens, espacenet) US6462258 (lens, espacenet) US6660911 (lens, espacenet) US2002144304 (lens, espacenet) US6919495 (lens, espacenet) US2003199681 (lens, espacenet) US6949696 (lens, espacenet) US2003199682 (lens, espacenet) US7112725 (lens, espacenet) US2005005332 (lens, espacenet) US7141722 (lens, espacenet) US2005022261 (lens, espacenet) JP2003516753 (lens, espacenet) EP2339005 (lens, espacenet)

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Method: QT-ELE-00-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 419 500 + 82 bp

Amplicon: 82 bp
1     gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgttc caaccacgtc tt 

View other 26489 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 4.54e-08
primerB 100.0 22 bp 0 0 82 61 3.55e-10
probe 100.0 22 bp 0 0 21 42 3.55e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.