JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_AX930592
Database:	nrnl1	Go to original record
Description:	Sequence 64 from Patent WO03087386. Sequence 58 from Patent WO2004024932. Sequence 66 from Patent WO2004024933. Sequence 66 from Patent WO2004024931. Sequence 58 from patent US 7238502. Sequence 66 from patent US 7282357. Sequence 66 from patent US 7381549. MetH. Process for the production of sulfur-containing fine chemicals. MetA. MetF. Methods for producing sulphurous fine chemicals. Method for the production by fermentation of sulphur-containing fine chemicals(METF). Method for zymotic production of fine chemicals containing sulphur (META). Method for zymotic production of fine chemicals containing sulphur (METY). Sequence 64 from patent US 7485444.
Taxon:	unidentified
Original size:	4323 bp
Patents:	WO03087386 (lens, espacenet) WO2004024932 (lens, espacenet) WO2004024933 (lens, espacenet) WO2004024931 (lens, espacenet) US7238502 (lens, espacenet) US2006003425 (lens, espacenet) US7282357 (lens, espacenet) US2006068476 (lens, espacenet) US7381549 (lens, espacenet) US2005260721 (lens, espacenet) JP2005522218 (lens, espacenet) US7485444 (lens, espacenet) US2006223149 (lens, espacenet)

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Method:	QL-ELE-00-002	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:	nptII

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1994	2208	+	215 bp

Amplicon:

215 bp

1     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
201   gccaggctca aggcg

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Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	21 bp	0	0	1	21	3.06e-09
primerB	100.0	21 bp	0	0	215	195	3.06e-09

Match details