Match details
| Record ID: |
NRN_BD170162 |
| Database: |
nrnl1 |
Go to original record |
| Description: |
Method of quantifying gene modification and standard molecule to be used therein. Sequence 75 from patent US 7709232. |
| Taxon: |
unidentified |
| Original size: |
239 bp |
| Patents: |
WO0234943 (lens, espacenet)
US7709232 (lens, espacenet)
|
View other 6 matches for this record
| Method: |
QT-TAX-GM-007 |
Go to GMOMETHODS record |
| Description: | Quantitative PCR method for detection of soybean lectin gene |
| Target: | soybean lectin |
View other 38 matches for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 0 |
0 |
122 |
239 |
- |
118 bp |
|
1 gccctctact ccacccccat ccacatttgg gacaaagaaa ccggtagcgt
51 tgccagcttc gccgcttcct tcaacttcac cttctatgcc cctgacacaa
101 aaaggcttgc agatgggc
View other 92 matches with an identical amplicon
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
19 bp |
0 |
0 |
1 |
19 |
1.99e-08 |
| primerB |
100.0 |
20 bp |
0 |
0 |
118 |
99 |
5.94e-09 |
| probe |
100.0 |
26 bp |
0 |
0 |
55 |
80 |
3.63e-12 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
