Match details
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Method:
QT-CON-00-005
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Quantitative PCR method for detection of the junction between the phosphinothricin N-acetyltransferase gene and the CaMV 35S terminator.
Target: pat-CaMV T-35S
View other 222 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
517
664
-
148 bp
1 gccagttagg ccagttaccc agatctgagt cgacctgcag gcatgccgct
51 gaaatcacca gtctctctct acaaatctat ctctctctat aataatgtgt
101 gagtagttcc cagataaggg aattagggtt cttatagggt ttcgctca
View other 31 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
21 bp
0
0
1
21
2.08e-09
primerB
100.0
24 bp
0
0
148
125
5.36e-11
probe
95.5
22 bp
0
1
36
56
1.03e-07
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.