JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_GX914744
Database:	nrnl1	Go to original record
Description:	Sequence 10652 from patent US 7868149.
Taxon:	unidentified
Original size:	63097 bp
Patents:	US7868149 (lens, espacenet)

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	34960	35575	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggcaacttc caaattcaga gaaaccctgg aattaaaaaa
101   ggggcaatcc tgagccaaat ccatgttttg agaaaacaag cggttctcga
151   actagaaccc aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ctagcggaac tcccttctaa
251   attagggaaa gaagggcttt cgaaatctaa tacacacgta tagatactgg
301   catagcaaac gattaatcac agaactcata tcataatata ggttctttaa
351   ttctttttta aaatgaaaat aggaatgatt atgaaataga aaatccataa
401   tttttttaga attggtgtga atccattcca atcgaatatt gagtaatcaa
451   atccttcaat tcataggttt tcgaaatctt tttaaaagcg gattaatcgg
501   acgaggataa agagagagtc ccattctaca tgtcaatact gacaacaatg
551   aaatttctag taaaaggaaa atccgtcgac tttctaagtc gcgagggttc
601   aagtccctct atcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details