Match details

Record ID: NRN_AX032992
Database: nrnl1 Go to original record
Description: Sequence 3 from Patent WO0044912. Manipulating isoprenoid expression.
Taxon: unidentified
Original size: 16032 bp
Patents: WO0044912 (lens, espacenet) JP2002535970 (lens, espacenet)

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Method: QL-ELE-00-006 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (L 00.00-31, 1998).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 4115 4334 - 220 bp

Amplicon: 220 bp
1     gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
51    acgttaagca tgtaataatt aacatgtaat gcacagatag gcctaacgct
101   tgtccaagat ctattcaggt gcatgacgtt atttatgaga tgggttttta
151   tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
201   tagcgcgcaa actaggataa  

View other 13 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 1.05e-08
primerB 100.0 20 bp 0 0 220 201 1.05e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.