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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_FB729833
Database: nrnl1 Go to original record
Description: Sequence 2 from Patent WO2007024866. Sequence 73 from Patent WO2007024782. Compositions Providing Tolerance to Multiple Herbicides and Methods of Use Thereof. Sequence 73 from patent US 7622641. Sequence 73 from patent US 7803992. Sequence 73 from patent US 7973218. Sequence 73 from patent US 8203033.
Taxon: unidentified
Original size: 534 bp
Patents: WO2007024866 (lens, espacenet) WO2007024866 (lens, espacenet) WO2007024782 (lens, espacenet) WO2007024782 (lens, espacenet) JP2009505654 (lens, espacenet) US7622641 (lens, espacenet) US2007079393 (lens, espacenet) US7803992 (lens, espacenet) US7973218 (lens, espacenet) US8203033 (lens, espacenet)

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Method: QL-ELE-00-017 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Barbau-Piednoir et al., 2014).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
2 0 417 491 + 75 bp
Amplicon: 75 bp 
1     aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
51    aatcccacta agcttcgcaa gaccc

View other 7 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 9.50e-11
primerB 90.0 20 bp 2 0 75 56 7.97e-06

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.