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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CS493961
Database: nrnl1 Go to original record
Description: Sequence 839 from Patent WO2006130156. Sequence 69 from Patent WO2006033708. Sequence 209 from patent US 7598429. Sequence 61 from patent US 7868229. Sequence 69 from patent US 7888558. Sequence 25 from patent US 7939715.
Taxon: unidentified
Original size: 574 bp
Patents: WO2006130156 (lens, espacenet) WO2006033708 (lens, espacenet) US7598429 (lens, espacenet) US2006195944 (lens, espacenet) US7868229 (lens, espacenet) US7888558 (lens, espacenet) US7939715 (lens, espacenet)

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Method: QT-ELE-00-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 371 452 + 82 bp
Amplicon: 82 bp 
1     gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgttc caaccacgtc tt

View other 26489 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 4.54e-08
primerB 100.0 22 bp 0 0 82 61 3.55e-10
probe 100.0 22 bp 0 0 21 42 3.55e-10

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.