Match details

Record ID: NRN_EA756592
Database: nrnl1 Go to original record
Description: Sequence 1 from patent US 7371848. TRANSGENIC CROP PLANTS WITH IMPROVED STRESS TOLERANCE. Sequence 1 from patent US 7838652. Sequence 1 from Patent EP2333079.
Taxon: unidentified
Original size: 612 bp
Patents: US7371848 (lens, espacenet) US2005283856 (lens, espacenet) JP2009540830 (lens, espacenet) US7838652 (lens, espacenet) EP2333079 (lens, espacenet)

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Method: QL-ELE-00-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Lipp et al.,2001).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
2 2 491 612 + 122 bp

Amplicon: 122 bp
1     ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
51    gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
101   aagttcattt catttggaga gg 

View other 99 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.82e-09
primerB 100.0 24 bp 0 0 122 99 4.62e-11


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.