Match details

Record ID: NRN_CS402030
Database: nrnl1 Go to original record
Description: Sequence 33 from Patent WO2006014316. Sequence 1 from Patent WO2007024866. Sequence 72 from Patent WO2007024782. Sequence 33 from patent US 7306946. Compositions Providing Tolerance to Multiple Herbicides and Methods of Use Thereof. Sequence 72 from patent US 7622641. Sequence 33 from patent US 7772370. Sequence 72 from patent US 7803992. Sequence 33 from patent US 7923599. Sequence 72 from patent US 7973218. Sequence 33 from patent US 8148510. Sequence 72 from patent US 8203033. Sequence 33 from Patent EP2113512.
Taxon: unidentified
Original size: 438 bp
Patents: WO2006014316 (lens, espacenet) WO2007024866 (lens, espacenet) WO2007024866 (lens, espacenet) WO2007024782 (lens, espacenet) WO2007024782 (lens, espacenet) US7306946 (lens, espacenet) US2006031962 (lens, espacenet) JP2009505654 (lens, espacenet) US7622641 (lens, espacenet) US2007079393 (lens, espacenet) US7772370 (lens, espacenet) US7803992 (lens, espacenet) US7923599 (lens, espacenet) US7973218 (lens, espacenet) US8148510 (lens, espacenet) US8203033 (lens, espacenet) EP2113512 (lens, espacenet)

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Method: QL-ELE-00-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim CaMV P-35S) (Waiblinger et al., 2008).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 334 415 + 82 bp

Amplicon: 82 bp
1     gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgttc caaccacgtc tt 

View other 26489 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 4.54e-08
primerB 100.0 22 bp 0 0 82 61 3.55e-10
probe 100.0 22 bp 0 0 21 42 3.55e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.