Match details

Record ID: NRN_DD278615
Database: nrnl1 Go to original record
Description: The reagent for measuring quantity of nucleic acid,and measuring method using this.
Taxon: unidentified
Original size: 128 bp

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Method: QT-TAX-GM-007 Go to GMOMETHODS record
Description:Quantitative PCR method for detection of soybean lectin gene
Target:soybean lectin

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 6 123 + 118 bp

Amplicon: 118 bp
1     gccctctact ccacccccat ccacatttgg gacaaagaaa ccggtagcgt
51    tgccagcttc gccgcttgct tcaacttcac cttctatgcc cctcacacaa
101   aaaggcttgc agatgggc 

View other 5 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 1.99e-08
primerB 100.0 20 bp 0 0 118 99 5.94e-09
probe 96.2 26 bp 1 0 55 80 1.69e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.