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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_DM044317
Database: nrnl1 Go to original record
Description: Method for increasing transformation efficiency of plants, comprising a cocultivation step of culturing plant tissues with cocultivation medium including 3,6-dichloro-o-anisic acid. Method for producing transformed plants by Agrobacterium without a selection step. Sequence 1 from patent US 8058514. Sequence 1 from patent US 8101820.
Taxon: unidentified
Original size: 12347 bp
Patents: WO2008105508 (lens, espacenet) WO2008105509 (lens, espacenet) US8058514 (lens, espacenet) US8101820 (lens, espacenet)

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Method: QL-ELE-00-006 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (L 00.00-31, 1998).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 3973 4152 + 180 bp
Amplicon: 180 bp 
1     gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
51    acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
101   tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
151   aaacaaaata tagcgcgcaa actaggataa

View other 29619 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 8.54e-09
primerB 100.0 20 bp 0 0 180 161 8.54e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.