Match details

Record ID: NRN_A78762
Database: nrnl1 Go to original record
Description: Sequence 23 from Patent EP0566525. Sequence 23 from patent US 5773700. Improvements in or relating to organic compounds. RECOMBINANT DNA WHICH CODING RNA OF ROSPOVIRUS, AND PLANT TRNSFORMED WITH THE GENE.
Taxon: unidentified
Original size: 446 bp
Patents: EP0566525 (lens, espacenet) US5773700 (lens, espacenet) JP2002223785 (lens, espacenet)

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Method: QT-ELE-00-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 181 262 + 82 bp

Amplicon: 82 bp
1     gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgttc caaccacgtc tt 

View other 26489 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 4.54e-08
primerB 100.0 22 bp 0 0 82 61 3.55e-10
probe 100.0 22 bp 0 0 21 42 3.55e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.