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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_A78762
Database: nrnl1 Go to original record
Description: Sequence 23 from Patent EP0566525. Sequence 23 from patent US 5773700. Improvements in or relating to organic compounds. RECOMBINANT DNA WHICH CODING RNA OF ROSPOVIRUS, AND PLANT TRNSFORMED WITH THE GENE.
Taxon: unidentified
Original size: 446 bp
Patents: EP0566525 (lens, espacenet) US5773700 (lens, espacenet) JP2002223785 (lens, espacenet)

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Method: QT-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Feinberg et al., 2005).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 257 335 + 79 bp
Amplicon: 79 bp 
1     cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat
51    gacgcacaat cccactatcc ttcgcaaga

View other 6569 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 22 bp 0 0 1 22 3.41e-10
primerB 100.0 22 bp 0 0 79 58 3.41e-10
probe 100.0 26 bp 0 0 31 56 2.52e-12

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.