Match details

Record ID: NRN_A78762
Database: nrnl1 Go to original record
Description: Sequence 23 from Patent EP0566525. Sequence 23 from patent US 5773700. Improvements in or relating to organic compounds. RECOMBINANT DNA WHICH CODING RNA OF ROSPOVIRUS, AND PLANT TRNSFORMED WITH THE GENE.
Taxon: unidentified
Original size: 446 bp
Patents: EP0566525 (lens, espacenet) US5773700 (lens, espacenet) JP2002223785 (lens, espacenet)

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Method: QL-ELE-00-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Lipp et al.,2001).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 254 376 + 123 bp

Amplicon: 123 bp
1     ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
51    gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
101   aagttcattt catttggaga gga 

View other 3472 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.84e-09
primerB 100.0 25 bp 0 0 123 99 1.30e-11


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.