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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CS804764
Database: nrnl1 Go to original record
Description: Sequence 101 from Patent WO2007044115. NOVEL IMMUNOADHESINS FOR TREATING AND PREVENTING VIRAL AND BACTERIAL DISEASES. IMPROVED CHIMERIC TOXIN PROTEINS AND CHIMERIC TOXIN RECEPTOR PROTEINS FOR TREATMENT AND PREVENTION OF ANTHRAX. JP 2010241817-A/44: NOVEL IMMUNOADHESINS FOR TREATING AND PREVENTING TOXICITY AND PATHOGEN-MEDIATED DISEASES.
Taxon: unidentified
Original size: 7129 bp
Patents: WO2007044115 (lens, espacenet) JP2005522998 (lens, espacenet) JP2009502205 (lens, espacenet) JP2010241817 (lens, espacenet)

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Method: QL-ELE-00-024 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of tE9 terminator (Debode et al., 2016).
Target:tE9

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 5211 5297 + 87 bp
Amplicon: 87 bp 
1     tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa
51    gagttaatga atgatatggt ccttttgttc attctca

View other 1026 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 1.11e-10
primerB 100.0 26 bp 0 0 87 62 2.79e-12
probe 100.0 36 bp 0 0 60 25 1.09e-17

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.