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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CS804763
Database: nrnl1 Go to original record
Description: Sequence 100 from Patent WO2007044115. NOVEL IMMUNOADHESINS FOR TREATING AND PREVENTING VIRAL AND BACTERIAL DISEASES. IMPROVED CHIMERIC TOXIN PROTEINS AND CHIMERIC TOXIN RECEPTOR PROTEINS FOR TREATMENT AND PREVENTION OF ANTHRAX. JP 2010241817-A/43: NOVEL IMMUNOADHESINS FOR TREATING AND PREVENTING TOXICITY AND PATHOGEN-MEDIATED DISEASES.
Taxon: unidentified
Original size: 6602 bp
Patents: WO2007044115 (lens, espacenet) JP2005522998 (lens, espacenet) JP2009502205 (lens, espacenet) JP2010241817 (lens, espacenet)

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Method: QL-CON-00-014 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the nos promoter and the neomycin phosphotransferase II gene
Target:P-nos-nptII

View other 1572 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 4773 4921 + 149 bp
Amplicon: 149 bp 
1     ttcccctcgg tatccaatta gagtctcata ttcactctca atccagatct
51    ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
101   cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatc

View other 25 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 1.83e-10
primerB 100.0 22 bp 0 0 149 128 6.20e-10
probe 100.0 25 bp 0 0 126 102 1.58e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.