Match details
Record ID:
NRN_AR110602
Database:
nrnl1
Go to original record
Description:
Sequence 9 from patent US 6114608. Sequence 9 from patent US 6229075. Sequence 9 from patent US 6232533. Sequence 9 from patent US 6232534. Sequence 9 from patent US 6342660. Sequence 9 from patent US 6399860. Sequence 9 from patent US 6573438.
Taxon:
unidentified
Original size:
7378 bp
Patents:
US6114608 (lens , espacenet )
US6229075 (lens , espacenet )
US6229075 (lens , espacenet )
US6232533 (lens , espacenet )
US6232533 (lens , espacenet )
US6232534 (lens , espacenet )
US6232534 (lens , espacenet )
US6342660 (lens , espacenet )
US6342660 (lens , espacenet )
US6399860 (lens , espacenet )
US6399860 (lens , espacenet )
US6573438 (lens , espacenet )
US6573438 (lens , espacenet )
View other 32 matches for this record
Method:
QT-CON-00-006
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Quantitative PCR method for detection of the junction between the intron 6 from maize alcohol dehydrogenase 1 gene and the synthetic cry1A(b) gene.
Target: IVS6-cryIAb
View other 14 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
983
1109
+
127 bp
1 aaaagaccac aacaagccgc ggatcctcta gagtcgacca tggacaacaa
51 cccaaacatc aacgaatgca ttccatacaa ctgcttgagt aacccagaag
101 ttgaagtact tggtggagaa cgcattg
View other 14 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
20 bp
0
0
1
20
6.42e-09
primerB
100.0
23 bp
0
0
127
105
1.55e-10
probe
100.0
27 bp
0
0
35
61
1.14e-12
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.