Match details

Record ID: NRN_AR110600
Database: nrnl1 Go to original record
Description: Sequence 7 from patent US 6114608. Sequence 7 from patent US 6229075. Sequence 7 from patent US 6232533. Sequence 7 from patent US 6232534. Sequence 7 from patent US 6342660. Sequence 7 from patent US 6399860. Sequence 7 from patent US 6573438.
Taxon: unidentified
Original size: 568 bp
Patents: US6114608 (lens, espacenet) US6229075 (lens, espacenet) US6229075 (lens, espacenet) US6232533 (lens, espacenet) US6232533 (lens, espacenet) US6232534 (lens, espacenet) US6232534 (lens, espacenet) US6342660 (lens, espacenet) US6342660 (lens, espacenet) US6399860 (lens, espacenet) US6399860 (lens, espacenet) US6573438 (lens, espacenet) US6573438 (lens, espacenet)

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Method: QT-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of phosphinothricin N-acetyltransferase gene (Weighard et al., 2004).
Target:pat

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 196 263 + 68 bp

Amplicon: 68 bp
1     ttgagggtgt tgtggctggt attgcttacg ctgggccctg gaaggctagg
51    aacgcttacg attggaca 

View other 1562 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 9.83e-10
primerB 100.0 21 bp 0 0 68 48 9.83e-10
probe 100.0 22 bp 0 0 44 23 2.90e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.