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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_AR110600
Database: nrnl1 Go to original record
Description: Sequence 7 from patent US 6114608. Sequence 7 from patent US 6229075. Sequence 7 from patent US 6232533. Sequence 7 from patent US 6232534. Sequence 7 from patent US 6342660. Sequence 7 from patent US 6399860. Sequence 7 from patent US 6573438.
Taxon: unidentified
Original size: 568 bp
Patents: US6114608 (lens, espacenet) US6229075 (lens, espacenet) US6229075 (lens, espacenet) US6232533 (lens, espacenet) US6232533 (lens, espacenet) US6232534 (lens, espacenet) US6232534 (lens, espacenet) US6342660 (lens, espacenet) US6342660 (lens, espacenet) US6399860 (lens, espacenet) US6399860 (lens, espacenet) US6573438 (lens, espacenet) US6573438 (lens, espacenet)

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Method: QL-ELE-00-025 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of pat gene; bar gene (partim pat) (Debode et al., 2016).
Target:pat

View other 1741 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 62 169 + 108 bp
Amplicon: 108 bp 
1     cgcggtttgt gatatcgtta accattacat tgagacgtct acagtgaact
51    ttaggacaga gccacaaaca ccacaagagt ggattgatga tctagagagg
101   ttgcaaga

View other 1557 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 22 bp 0 0 1 22 4.75e-10
primerB 100.0 25 bp 0 0 108 84 1.20e-11
probe 100.0 29 bp 0 0 53 81 8.17e-14

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.