Match details
Record ID:
NRN_A95052
Database:
nrnl1
Go to original record
Description:
Sequence 9 from Patent WO9929880. Sequence 8 from patent US 6603062. Method of constructing transgenic plant accompanied by biosynthesis of modified 5-aminolevulinic acid and method of identifying 5-aminolevulinic acid synthesis effector.
Taxon:
unidentified
Original size:
4186 bp
Patents:
WO9929880 (lens , espacenet )
US6603062 (lens , espacenet )
US6603062 (lens , espacenet )
JP2001526041 (lens , espacenet )
View other 12 matches for this record
Method:
QT-CON-00-005
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Quantitative PCR method for detection of the junction between the phosphinothricin N-acetyltransferase gene and the CaMV 35S terminator.
Target: pat-CaMV T-35S
View other 222 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
345
474
-
130 bp
1 gccagttagg ccagttaccc agatctgacg ctgaaatcac cagtctctct
51 ctacaaatct atctctctct ataataatgt gtgagtagtt cccagataag
101 ggaattaggg ttcttatagg gtttcgctca
View other 3 matches with an identical amplicon
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
21 bp
0
0
1
21
1.95e-09
primerB
100.0
24 bp
0
0
130
107
4.68e-11
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.