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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_JA687154
Database: nrnl1 Go to original record
Description: Sequence 6 from Patent WO2011153186.
Taxon: unidentified
Original size: 6126 bp
Patents: WO2011153186 (lens, espacenet)

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Method: QT-EVE-BN-010 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of oilseed rape event MON88302 (Savini et al., 2013).
Target:MON88302

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 735 835 + 101 bp
Amplicon: 101 bp 
1     tccttgaacc ttattttata gtgcacaaaa ccttttagtc atcatgttgt
51    accacttcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
101   a

View other 11 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 27 bp 0 0 1 27 9.51e-13
primerB 100.0 24 bp 0 0 101 78 3.82e-11
probe 100.0 30 bp 0 0 36 65 2.21e-14

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.