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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_AR014742
Database: nrnl1 Go to original record
Description: Sequence 9 from patent US 5773701. Sequence 9 from patent US 5792937. Sequence 1 from patent US 5804425. Sequence 4 from patent US 5986175. Sequence 1 from patent US 7183110. Sequence 4 from patent US 5503999. Sequence 17 from patent US 5512466. Sequence 5 from patent US 5589612. Sequence 1 from patent US 5633435. Sequence 1 from patent US 5627061. Sequence 17 from patent US 5702933.
Taxon: unidentified
Original size: 597 bp
Patents: US5773701 (lens, espacenet) US5792937 (lens, espacenet) US5804425 (lens, espacenet) US5986175 (lens, espacenet) US7183110 (lens, espacenet) US2002168680 (lens, espacenet) US5503999 (lens, espacenet) US5512466 (lens, espacenet) US5589612 (lens, espacenet) US5633435 (lens, espacenet) US5627061 (lens, espacenet) US5702933 (lens, espacenet)

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Method: QL-ELE-00-010 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Figwort Mosaic Virus 35S promoter (Pan et al., 2007).
Target:P-FMV

View other 425 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 140 335 + 196 bp
Amplicon: 196 bp 
1     aagcctcaac aaggtcaggg tacagagtct ccaaaccatt agccaaaagc
51    tacaggagat caatgaagaa tcttcaatca aagtaaacta ctgttccagc
101   acatgcatca tggtcagtaa gtttcagaaa aagacatcca ccgaagactt
151   aaagttagtg ggcatctttg aaagtaatct tgtcaacatc gagcag

View other 417 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 1.03e-07
primerB 100.0 18 bp 0 0 196 179 1.03e-07

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.