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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_GP056454
Database: nrnl1 Go to original record
Description: Sequence 16 from patent US 7481998. Sequence 16 from patent US 7501114. Sequence 16 from patent US 7687058. Sequence 16 from patent US 7713522. Sequence 16 from patent US 7803365. Sequence 16 from patent US 8048410. Sequence 23 from patent US 8048410. Sequence 16 from patent US 8236300. Sequence 23 from patent US 8236300. Sequence 16 from patent US 8242095. Sequence 23 from patent US 8242095. Sequence 16 from Patent EP2099438. Sequence 16 from Patent EP2393497. Sequence 23 from Patent EP2393497.
Taxon: unidentified
Original size: 30756 bp
Patents: US7481998 (lens, espacenet) US2007212334 (lens, espacenet) US7501114 (lens, espacenet) US2007184027 (lens, espacenet) US7687058 (lens, espacenet) US7713522 (lens, espacenet) US7803365 (lens, espacenet) US8048410 (lens, espacenet) US8048410 (lens, espacenet) US8236300 (lens, espacenet) US8236300 (lens, espacenet) US8242095 (lens, espacenet) US8242095 (lens, espacenet) EP2099438 (lens, espacenet) EP2393497 (lens, espacenet) EP2393497 (lens, espacenet)

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Method: QL-ELE-00-003 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (BVL L 00.00-31, 1998)
Target:nptII

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 29451 29623 + 173 bp
Amplicon: 173 bp 
1     ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
51    ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
101   gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
151   cggtcttgtc gatcaggatg atc

View other 57974 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 16 bp 0 0 1 16 1.08e-06
primerB 100.0 16 bp 0 0 173 158 1.08e-06

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.