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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_GY009187
Database: nrnl1 Go to original record
Description: Sequence 40 from patent US 7883882. Sequence 40 from patent US 7935515. Sequence 40 from patent US 8187860. Sequence 40 from patent US 8222010. Sequence 40 from patent US 8268610. JP 2012510275-A/25: PRODUCTION OF TAILORED OILS IN HETEROTROPHIC MICROORGANISMS. JP 2012513743-A/25: MANUFACTURING OF TAILORED OILS IN RECOMBINANT HETEROTROPHIC MICROORGANISMS.
Taxon: unidentified
Original size: 2571 bp
Patents: US7883882 (lens, espacenet) US7935515 (lens, espacenet) US8187860 (lens, espacenet) US8222010 (lens, espacenet) US8268610 (lens, espacenet) JP2012510275 (lens, espacenet) JP2012513743 (lens, espacenet)

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Method: QL-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:nptII

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1649 1863 + 215 bp
Amplicon: 215 bp 
1     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
201   gccaggctca aggcg

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Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 3.06e-09
primerB 100.0 21 bp 0 0 215 195 3.06e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.