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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_HD087566
Database: nrnl1 Go to original record
Description: Sequence 15 from Patent WO2010077890. Sequence 16 from Patent WO2011046772. Sequence 48 from Patent WO2011136909. Sequence 15 from Patent EP2511374. Sequence 16 from Patent WO2012087903.
Taxon: unidentified
Original size: 50905 bp
Patents: WO2010077890 (lens, espacenet) WO2011046772 (lens, espacenet) WO2011136909 (lens, espacenet) EP2511374 (lens, espacenet) WO2012087903 (lens, espacenet)

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Method: QL-ELE-00-026 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of pat gene; bar gene (partim bar) (Debode et al., 2016).
Target:bar

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 39057 39116 + 60 bp
Amplicon: 60 bp 
1     acaagcacgg tcaacttccg taccgagccg caggaaccgc aggactggac
51    ggacgacctc

View other 85 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 1.06e-08
primerB 94.4 18 bp 1 0 60 43 1.65e-06
probe 100.0 18 bp 0 0 21 38 3.56e-08

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.