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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_AX441381
Database: nrnl1 Go to original record
Description: Sequence 12 from Patent EP1209237. Sequence 12 from Patent EP1213356. Sequence 12 from patent US 5859336. Sequence 12 from patent US 6075185. Sequence 12 from patent US 6720488. Sequence 12 from patent US 5625136.
Taxon: unidentified
Original size: 3546 bp
Patents: EP1209237 (lens, espacenet) EP1213356 (lens, espacenet) US5859336 (lens, espacenet) US6075185 (lens, espacenet) US6720488 (lens, espacenet) US2003046726 (lens, espacenet) US5625136 (lens, espacenet)
Method: QT-ELE-00-003 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of synthetic cry1A(b) gene (ISO/FDIS 21570:2005).
Target:cryIAb

View other 199 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 103 231 + 129 bp
Amplicon: 129 bp 
1     cccatcgaca tcagcctgag cctgacccag ttcctgctga gcgagttcgt
51    gcccggcgcc ggcttcgtgc tgggcctggt ggacatcatc tggggcatct
101   tcggccccag ccagtgggac gccttcctg

View other 183 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.93e-09
primerB 100.0 20 bp 0 0 129 110 6.52e-09
probe 100.0 21 bp 0 0 86 66 1.93e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.