Match details

Record ID: NRN_GP466869
Database: nrnl1 Go to original record
Description: Sequence 19 from patent US 7554007. Sequence 31 from patent US 7812218. Sequence 46 from patent US 7906705. Sequence 120 from patent US 7910800. Sequence 841 from patent US 8168857. Sequence 2825 from Patent WO2009083958. Sequence 31 from Patent EP2186899. Sequence 120 from Patent EP1945021. Sequence 841 from Patent EP2096909. Sequence 921 from Patent EP2154946. Sequence 1546 from Patent EP2183371. Sequence 31 from Patent EP2343373. Sequence 841 from Patent EP2383345.
Taxon: unidentified
Original size: 877 bp
Patents: US7554007 (lens, espacenet) US2006123516 (lens, espacenet) US7812218 (lens, espacenet) US7906705 (lens, espacenet) US7910800 (lens, espacenet) US8168857 (lens, espacenet) WO2009083958 (lens, espacenet) EP2186899 (lens, espacenet) EP1945021 (lens, espacenet) EP2096909 (lens, espacenet) EP2154946 (lens, espacenet) EP2183371 (lens, espacenet) EP2343373 (lens, espacenet) EP2383345 (lens, espacenet)

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Method: QL-ELE-00-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim CaMV P-35S) (Waiblinger et al., 2008).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 660 741 + 82 bp

Amplicon: 82 bp
1     gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgttc caaccacgtc tt 

View other 26489 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 4.54e-08
primerB 100.0 22 bp 0 0 82 61 3.55e-10
probe 100.0 22 bp 0 0 21 42 3.55e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.