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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_GY508888
Database: nrnl1 Go to original record
Description: Sequence 33 from patent US 8052968. Sequence 33 from patent US 8066984. Sequence 33 from Patent WO2008100292. Sequence 33 from Patent WO2009139921.
Taxon: unidentified
Original size: 4095 bp
Patents: US8052968 (lens, espacenet) US8066984 (lens, espacenet) WO2008100292 (lens, espacenet) WO2009139921 (lens, espacenet)
Method: QL-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:nptII

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 1796 2010 + 215 bp
Amplicon: 215 bp 
1     cccaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
201   gccaggctca aggcg

View other 841 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 3 21 3.95e-08
primerB 100.0 21 bp 0 0 215 195 3.06e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.