European Commission logo
Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_GZ197698
Database: nrnl1 Go to original record
Description: Sequence 15 from patent US 8133723. Sequence 15 from Patent EP2217064. JP 2011506272-A/8: NOVEL VACCINES AGAINST MULTIPLE SUBTYPES OF INFLUENZA VIRUS.
Taxon: unidentified
Original size: 4733 bp
Patents: US8133723 (lens, espacenet) EP2217064 (lens, espacenet) JP2011506272 (lens, espacenet)

View other 1 match for this record

Method: QL-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:nptII

View other 59426 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 3274 3488 + 215 bp
Amplicon: 215 bp 
1     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
201   gccaggctca aggcg

View other 57490 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 3.06e-09
primerB 100.0 21 bp 0 0 215 195 3.06e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.