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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_HC319826
Database: nrnl1 Go to original record
Description: Sequence 50 from Patent WO2010003007. Sequence 50 from Patent WO2010031077. Sequence 50 from Patent WO2010031062. Sequence 50 from Patent WO2010031079. Sequence 50 from Patent WO2010031076. Sequence 50 from Patent WO2010031068. Sequence 16 from Patent WO2010078457. Sequence 50 from Patent EP2235190. JP 2011505841-A/50: COMPOSITIONS AND METHODS FOR PRODUCING ISOPRENE. Sequence 50 from Patent EP2307552.
Taxon: unidentified
Original size: 6912 bp
Patents: WO2010003007 (lens, espacenet) WO2010031077 (lens, espacenet) WO2010031062 (lens, espacenet) WO2010031079 (lens, espacenet) WO2010031076 (lens, espacenet) WO2010031068 (lens, espacenet) WO2010078457 (lens, espacenet) EP2235190 (lens, espacenet) JP2011505841 (lens, espacenet) EP2307552 (lens, espacenet)

View other 1 match for this record

Method: QL-ELE-00-003 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (BVL L 00.00-31, 1998)
Target:nptII

View other 58813 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 5386 5558 + 173 bp
Amplicon: 173 bp 
1     ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
51    ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
101   gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
151   cggtcttgtc gatcaggatg atc

View other 57974 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 16 bp 0 0 1 16 1.08e-06
primerB 100.0 16 bp 0 0 173 158 1.08e-06

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.