Match details

Record ID: NRN_AX063413
Database: nrnl1 Go to original record
Description: Sequence 5 from Patent WO0100833.
Taxon: unidentified
Original size: 7599 bp
Patents: WO0100833 (lens, espacenet)

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Method: QL-CON-00-015 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the CaMV35S promoter and the bar gene (verified by the EURL GMFF in the context of Commission Decision 2006/578/EC).
Target:CaMV P-35S-bar

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 5837 5959 + 123 bp

Amplicon: 123 bp
1     tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
51    gaggacacgc tgaaatcacc agtctctctc tacaaatcgg atccatgagc
101   ccagaacgac gcccggccga cat 

View other 6 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.84e-09
primerB 100.0 22 bp 0 0 123 102 5.44e-10
probe 100.0 25 bp 0 0 22 46 1.30e-11


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.