Match details
Record ID: NRN_AX033509
Database:
nrnl1
Go to original record
Description:
Sequence 17 from Patent DE19906169. Test kit and method for quantitatively detecting genetically modi-fied DNA in foodstuff by means of fluorescence-coupled PCR.
Taxon:
unidentified
Original size:
250 bp
Patents:
DE19906169 (lens , espacenet )
JP2002536024 (lens , espacenet )
View other 7 matches for this record
Method: QT-TAX-GM-005
Go to GMOMETHODS record
Description: Quantitative PCR method for detection of soybean lectin gene
Target: soybean lectin
View other 20 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
117
190
+
74 bp
1 ccagcttcgc cgcttccttc aacttcacct tctatgcccc tgacacaaaa
51 aggcttgcag atgggcttgc cttc
View other 25 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
20 bp
0
0
1
20
3.63e-09
primerB
100.0
23 bp
0
0
74
52
9.36e-11
probe
100.0
24 bp
0
0
23
46
2.75e-11
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
