Match details

Record ID: NRN_FW567550
Database: nrnl1 Go to original record
Description: PRODUCTION OF OIL IN MICROORGANISMS. Sequence 5 from Patent EP2152849. Sequence 5 from Patent EP2351845.
Taxon: unidentified
Original size: 2435 bp
Patents: JP2010528627 (lens, espacenet) EP2152849 (lens, espacenet) EP2351845 (lens, espacenet)

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Method: QL-ELE-00-005 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (ISO/FDIS 21569:2005).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 530 724 + 195 bp

Amplicon: 195 bp
1     gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
51    gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
101   cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
151   gtgatatctc cactgacgta agggatgacg cacaatccca ctatc 

View other 3571 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 94.7 19 bp 1 0 1 19 1.44e-06
primerB 100.0 20 bp 0 0 195 176 9.28e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.