JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_GX641316
Database:	nrnl1	Go to original record
Description:	Sequence 31 from patent US 7812223. Sequence 31 from patent US 8158859. Sequence 1 from Patent WO2009058947. Sequence 1 from Patent WO2009061776. Sequence 31 from Patent WO2009073605. Sequence 1 from Patent WO2009126359. Sequence 1 from Patent WO2009132057. Sequence 1 from Patent WO2010006010. Sequence 1 from Patent WO2010011907. Sequence 1 from Patent WO2010019872. Sequence 1 from Patent WO2010053621. Sequence 1 from Patent WO2010077890. Sequence 1 from Patent WO2010104848. Sequence 1 from Patent WO2010144276. Sequence 1 from Patent WO2011008510. Sequence 1 from Patent WO2011046772. Sequence 1 from Patent EP2511374. Sequence 1 from Patent WO2012087903.
Taxon:	unidentified
Original size:	18491 bp
Patents:	US7812223 (lens, espacenet) US8158859 (lens, espacenet) WO2009058947 (lens, espacenet) WO2009061776 (lens, espacenet) WO2009073605 (lens, espacenet) WO2009126359 (lens, espacenet) WO2009132057 (lens, espacenet) WO2010006010 (lens, espacenet) WO2010011907 (lens, espacenet) WO2010019872 (lens, espacenet) WO2010053621 (lens, espacenet) WO2010077890 (lens, espacenet) WO2010104848 (lens, espacenet) WO2010144276 (lens, espacenet) WO2011008510 (lens, espacenet) WO2011046772 (lens, espacenet) EP2511374 (lens, espacenet) WO2012087903 (lens, espacenet)

Method:	QT-ELE-00-004	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter
Target:	CaMV P-35S

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	179	260	-	82 bp

Amplicon:

82 bp

1     gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgttc caaccacgtc tt

Primers and probe alignment:

Primer/Probe	% Identity	Length	From	To	Score
primerA	100.0	18 bp	1	18	4.54e-08
primerB	100.0	22 bp	82	61	3.55e-10
probe	100.0	22 bp	21	42	3.55e-10

Match details