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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_FW503946
Database: nrnl1 Go to original record
Description: Delta-8 Desaturases and Their Use in Making Polyunsaturated Fatty Acids. Sequence 148 from Patent WO2008100545. Sequence 10 from Patent WO2008124194. Sequence 10 from patent US 7790156. Sequence 148 from patent US 8013215. Sequence 10 from patent US 8188338. Sequence 10 from Patent WO2008124806.
Taxon: unidentified
Original size: 4116 bp
Patents: JP2010523147 (lens, espacenet) WO2008100545 (lens, espacenet) WO2008124194 (lens, espacenet) US7790156 (lens, espacenet) US8013215 (lens, espacenet) US8188338 (lens, espacenet) WO2008124806 (lens, espacenet)

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Method: QL-ELE-00-003 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (BVL L 00.00-31, 1998)
Target:nptII

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2144 2316 - 173 bp
Amplicon: 173 bp 
1     ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
51    ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
101   gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
151   cggtcttgtc gatcaggatg atc

View other 57974 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 16 bp 0 0 1 16 1.08e-06
primerB 100.0 16 bp 0 0 173 158 1.08e-06

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.