Match details

Record ID: NRN_GM702761
Database: nrnl1 Go to original record
Description: Sequence 4914 from Patent WO2008088422. Sequence 4914 from patent US 7803769. Sequence 4914 from patent US 7820623. Sequence 4914 from patent US 7825093. Sequence 4914 from patent US 7834164. Sequence 4914 from patent US 7910102. Sequence 4914 from patent US 8043829.
Taxon: unidentified
Original size: 6587 bp
Patents: WO2008088422 (lens, espacenet) US7803769 (lens, espacenet) US7820623 (lens, espacenet) US7825093 (lens, espacenet) US7834164 (lens, espacenet) US7910102 (lens, espacenet) US8043829 (lens, espacenet)

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Method: QL-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:nptII

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 3165 3379 - 215 bp

Amplicon: 215 bp
1     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
201   gccaggctca aggcg 

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Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 3.06e-09
primerB 100.0 21 bp 0 0 215 195 3.06e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.