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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_GM961482
Database: nrnl1 Go to original record
Description: Sequence 8436 from Patent WO2008142034. Sequence 14390 from Patent WO2009037279. Sequence 6069 from Patent WO2009037329. Sequence 2905 from Patent WO2009077611. Sequence 16 from Patent WO2009106596. Sequence 46 from Patent WO2010020654. Sequence 46 from Patent WO2010034672. Sequence 46 from Patent WO2010046221.
Taxon: unidentified
Original size: 8885 bp
Patents: WO2008142034 (lens, espacenet) WO2009037279 (lens, espacenet) WO2009037329 (lens, espacenet) WO2009077611 (lens, espacenet) WO2009106596 (lens, espacenet) WO2010020654 (lens, espacenet) WO2010034672 (lens, espacenet) WO2010046221 (lens, espacenet)

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Method: QL-ELE-00-011 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator.
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2127 2210 + 84 bp
Amplicon: 84 bp 
1     catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
51    cgcaattata catttaatac gcgatagaaa acaa

View other 30799 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 25 bp 0 0 1 25 9.20e-12
primerB 100.0 25 bp 0 0 84 60 9.20e-12
probe 100.0 28 bp 0 0 28 55 2.28e-13

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.