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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_DI017219
Database: nrnl1 Go to original record
Description: Porcine uroplakin II promoter and the production method of usefulproteins using said promoter. Porcine uroplakin II promoter and the production method of useful proteins using said promoter. JP 2010535496-A/4: Mammary gland-specific human erythropoietin expression vector, transgenic animal and method for producing human erythropoietin using same. Sequence 5 from patent US 7416886. Sequence 4 from Patent EP2176414.
Taxon: unidentified
Original size: 1916 bp
Patents: JP2006505267 (lens, espacenet) JP2010535496 (lens, espacenet) US7416886 (lens, espacenet) US2006236412 (lens, espacenet) EP2176414 (lens, espacenet)

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Method: QL-ELE-00-003 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (BVL L 00.00-31, 1998)
Target:nptII

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 838 1010 + 173 bp
Amplicon: 173 bp 
1     ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
51    ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
101   gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
151   cggtcttgtc gatcaggatg atc

View other 57974 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 16 bp 0 0 1 16 1.08e-06
primerB 100.0 16 bp 0 0 173 158 1.08e-06

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.