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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_GM715929
Database: nrnl1 Go to original record
Description: Sequence 62 from Patent WO2008100552. Sequence 56 from Patent WO2009029739. Sequence 62 from patent US 7960613. Sequence 43 from Patent WO2009058947. Sequence 29 from Patent WO2009067569. Sequence 56 from Patent WO2010011907. Sequence 44 from Patent WO2010019872.
Taxon: unidentified
Original size: 12856 bp
Patents: WO2008100552 (lens, espacenet) WO2009029739 (lens, espacenet) US7960613 (lens, espacenet) WO2009058947 (lens, espacenet) WO2009067569 (lens, espacenet) WO2010011907 (lens, espacenet) WO2010019872 (lens, espacenet)

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Method: QL-ELE-00-014 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of phosphinothricin N-acetyltransferase gene (Grohmann et al., 2009).
Target:bar

View other 2622 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 11150 11209 + 60 bp
Amplicon: 60 bp 
1     acaagcacgg tcaacttccg taccgagccg caggaaccgc aggactggac
51    ggacgacctc

View other 85 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 1.06e-08
primerB 94.4 18 bp 1 0 60 43 1.65e-06
probe 100.0 18 bp 0 0 21 38 3.56e-08

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.