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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_FB667537
Database: nrnl1 Go to original record
Description: Sequence 59 from Patent WO2007141189. Sequence 313 from Patent EP1953235. Sequence 59 from patent US 8273952. Sequence 68242 from Patent EP2096177. Sequence 68242 from Patent EP2199304.
Taxon: unidentified
Original size: 13170 bp
Patents: WO2007141189 (lens, espacenet) WO2007141189 (lens, espacenet) EP1953235 (lens, espacenet) US8273952 (lens, espacenet) EP2096177 (lens, espacenet) EP2199304 (lens, espacenet)

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Method: QL-ELE-00-011 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator.
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 6901 6984 + 84 bp
Amplicon: 84 bp 
1     catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
51    cgcaattata catttaatac gcgatagaaa acaa

View other 30799 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 25 bp 0 0 1 25 9.20e-12
primerB 100.0 25 bp 0 0 84 60 9.20e-12
probe 100.0 28 bp 0 0 28 55 2.28e-13

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.