Match details

Record ID: NRN_GM979671
Database: nrnl1 Go to original record
Description: Sequence 75 from Patent WO2008147935. Sequence 75 from patent US 8143473. Sequence 75 from patent US 8143476. Sequence 75 from patent US 8153859. Sequence 75 from Patent WO2009143401. Sequence 75 from Patent WO2009143398.
Taxon: unidentified
Original size: 11693 bp
Patents: WO2008147935 (lens, espacenet) US8143473 (lens, espacenet) US8143476 (lens, espacenet) US8153859 (lens, espacenet) WO2009143401 (lens, espacenet) WO2009143398 (lens, espacenet)

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Method: QL-ELE-00-017 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Barbau-Piednoir et al., 2014).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 6036 6110 - 75 bp

Amplicon: 75 bp
1     aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac
51    aatcccacta tccttcgcaa gaccc 

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Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.23e-09
primerB 100.0 20 bp 0 0 75 56 3.68e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.