Match details

Record ID: NRN_GM961483
Database: nrnl1 Go to original record
Description: Sequence 8437 from Patent WO2008142034. Sequence 14395 from Patent WO2009037279. Sequence 6079 from Patent WO2009037329. Sequence 2906 from Patent WO2009077611. Sequence 26 from Patent WO2009106596. Sequence 56 from Patent WO2010020654. Sequence 56 from Patent WO2010034672. Sequence 7 from Patent WO2010037714. Sequence 56 from Patent WO2010046221.
Taxon: unidentified
Original size: 9043 bp
Patents: WO2008142034 (lens, espacenet) WO2009037279 (lens, espacenet) WO2009037329 (lens, espacenet) WO2009077611 (lens, espacenet) WO2009106596 (lens, espacenet) WO2010020654 (lens, espacenet) WO2010034672 (lens, espacenet) WO2010037714 (lens, espacenet) WO2010046221 (lens, espacenet)

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Method: QL-ELE-00-026 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of pat gene; bar gene (partim bar) (Debode et al., 2016).
Target:bar

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 634 693 - 60 bp

Amplicon: 60 bp
1     acaagcacgg tcaacttccg taccgagccg caggaaccgc aggagtggac
51    ggacgacctc  

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Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 1.06e-08
primerB 100.0 18 bp 0 0 60 43 3.56e-08
probe 100.0 18 bp 0 0 21 38 3.56e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.