Match details

Record ID: NRN_GM716097
Database: nrnl1 Go to original record
Description: Sequence 125 from Patent WO2008100545. Sequence 276 from Patent WO2008124048. Sequence 60 from Patent WO2009046006. Sequence 60 from patent US 7893324. Sequence 125 from patent US 8013215. Sequence 167 from patent US 8143476. Sequence 60 from patent US 8293976. Sequence 167 from Patent WO2009143398.
Taxon: unidentified
Original size: 7497 bp
Patents: WO2008100545 (lens, espacenet) WO2008124048 (lens, espacenet) WO2009046006 (lens, espacenet) US7893324 (lens, espacenet) US8013215 (lens, espacenet) US8143476 (lens, espacenet) US8293976 (lens, espacenet) WO2009143398 (lens, espacenet)

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Method: QL-ELE-00-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim CaMV P-35S) (Waiblinger et al., 2008).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2449 2530 + 82 bp

Amplicon: 82 bp
1     gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgttc caaccacgtc tt 

View other 26489 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 4.54e-08
primerB 100.0 22 bp 0 0 82 61 3.55e-10
probe 100.0 22 bp 0 0 21 42 3.55e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.